Review




Structured Review

Promega glosensor camp reporter plasmid p22f
Glosensor Camp Reporter Plasmid P22f, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glosensor+-22f+camp+plasmid/glosensor+camp+reporter+plasmid+p22f/pm40368898-308-5-10
Average 90 stars, based on 1 article reviews
glosensor camp reporter plasmid p22f - by Bioz Stars, 2026-09
90/100 stars

Images

Related Articles

other:

Article Title: MC4R Variants Modulate α-MSH and Setmelanotide Induced Cellular Signaling at Multiple Levels
Article Snippet: For the GloSensor cAMP assay, pcDNA3.1(+) expression plasmid containing WT or variant MC4R and the GloSensor 22F plasmid (Promega, USA) were used to measure cAMP accumulation.

Article Title: An shRNA screen in primary human beta cells identifies the serotonin 1F receptor as a negative regulator of survival during transplant
Article Snippet: Briefly, HEK293T cells were transiently transfected with the Glosensor-cAMP plasmid (Promega) with or without 5-HT 1F or 5-HT 1A cDNA.

Article Title: Adenosine receptor modulators for the treatment of circadian rhythm disorders
Article Snippet: Cells were transfected with siRNAs (typically at 50 nM concentrations, sequences as previously listed), the CRE::Luc (pGL4.29[luc2P/CRE/Hygro] Vector, encoding Luc2P luciferin under the control of a strong cAMP response element (CRE) promoter containing 3 CRE elements within its sequence; Promega) reporter vector or cAMP-GLOSensor plasmid (Promega) as per manufacturer's instructions using either Lipofectamine RNAimax (Invitrogen) or Lipofectamine3000 (Invitrogen).

Expressing:

Article Title: Decoding Molecular Mechanism Underlying Human Olfactory Receptor OR8D1 Activation by Sotolone Enantiomers.
Article Snippet: Sotolone, a chiral compound, plays an important role in the food industry.. Herein, (R)-/(S)-sotolone were separated to determine their odor characteristics and thresholds in air (R-form: smoky, burned, herb, and green aroma, 0.0514 μg/m3; S-form: sweet, milk, acid, and nutty aroma, 0.0048 μg/m3).. OR8D1 responses to (R)-/(S)-sotolone were detected in a HEK293 cell-based luminescence assay. (S)-Sotolone was a more potent agonist than (R)-sotolone (EC50 values of 84.98 ± 1.05 and 167.20 ± 0.25 μmol/L, respectively).

Article Title: Prolonging parathyroid hormone analog action in vitro and in vivo through peptide lipidation
Article Snippet: .. Functional assays were performed in stably transfected cell lines derived from HEK-293 cells (ATCC Cat. # CRL-1573) and expressing the glosensor cAMP reporter (plasmid p22f, Promega) along with either the human PTH1R (GP-2.3 cells), pHL2-hPTH1R, in which pHluorin2, a pH-sensitive GFP variant, is inserted into the E2 region of the hPTH1R’s ECD (GPG-10 cells), PTH1R-delNt yfp , in which the receptor’s N-terminal extracellular domain (ECD) is replaced by yellow fluorescent protein (GD-5y cells), or β-Arrestin2 yfp (GBR-24 cells). .. Assays were also performed in SGS-72 cells, which are derived from the human osteoblastic cell line Saos-2 (ATCC Cat. # HTB-85) and stably express glosensor.

Article Title: Prolonging parathyroid hormone analog action in vitro and in vivo through peptide lipidation.
Article Snippet: .. # CRL-1573) and expressing the glosensor cAMP reporter (plasmid p22f, Promega) along with either the human PTH1R (GP-2.3 cells), pHL2-hPTH1R, in which pHluorin2, a pH-sensitive GFP variant, is inserted into the E2 region of the hPTH1R’s ECD (GPG-10 cells), PTH1R-delNtyfp, in which the receptor’s N-terminal extracellular domain (ECD) is replaced by yellow fluorescent protein (GD-5y cells), or β-Arrestin2yfp (GBR-24 cells). .. Assays were also performed in SGS-72 cells, which are derived from the humanosteoblastic cell line Saos-2 (ATCC Cat. # HTB-85) and stably express glosensor.

Plasmid Preparation:

Article Title: Decoding Molecular Mechanism Underlying Human Olfactory Receptor OR8D1 Activation by Sotolone Enantiomers.
Article Snippet: Sotolone, a chiral compound, plays an important role in the food industry.. Herein, (R)-/(S)-sotolone were separated to determine their odor characteristics and thresholds in air (R-form: smoky, burned, herb, and green aroma, 0.0514 μg/m3; S-form: sweet, milk, acid, and nutty aroma, 0.0048 μg/m3).. OR8D1 responses to (R)-/(S)-sotolone were detected in a HEK293 cell-based luminescence assay. (S)-Sotolone was a more potent agonist than (R)-sotolone (EC50 values of 84.98 ± 1.05 and 167.20 ± 0.25 μmol/L, respectively).

Article Title: Prolonging parathyroid hormone analog action in vitro and in vivo through peptide lipidation
Article Snippet: .. Functional assays were performed in stably transfected cell lines derived from HEK-293 cells (ATCC Cat. # CRL-1573) and expressing the glosensor cAMP reporter (plasmid p22f, Promega) along with either the human PTH1R (GP-2.3 cells), pHL2-hPTH1R, in which pHluorin2, a pH-sensitive GFP variant, is inserted into the E2 region of the hPTH1R’s ECD (GPG-10 cells), PTH1R-delNt yfp , in which the receptor’s N-terminal extracellular domain (ECD) is replaced by yellow fluorescent protein (GD-5y cells), or β-Arrestin2 yfp (GBR-24 cells). .. Assays were also performed in SGS-72 cells, which are derived from the human osteoblastic cell line Saos-2 (ATCC Cat. # HTB-85) and stably express glosensor.

Article Title: Prolonging parathyroid hormone analog action in vitro and in vivo through peptide lipidation.
Article Snippet: .. # CRL-1573) and expressing the glosensor cAMP reporter (plasmid p22f, Promega) along with either the human PTH1R (GP-2.3 cells), pHL2-hPTH1R, in which pHluorin2, a pH-sensitive GFP variant, is inserted into the E2 region of the hPTH1R’s ECD (GPG-10 cells), PTH1R-delNtyfp, in which the receptor’s N-terminal extracellular domain (ECD) is replaced by yellow fluorescent protein (GD-5y cells), or β-Arrestin2yfp (GBR-24 cells). .. Assays were also performed in SGS-72 cells, which are derived from the humanosteoblastic cell line Saos-2 (ATCC Cat. # HTB-85) and stably express glosensor.

Article Title: Pharmacological modulation of MRAP2 protein on murine melatonin receptor signaling
Article Snippet: .. The pGloSensor-22F cAMP plasmid and GloSensor cAMP reagent were procured from Promega (Madison, WI, USA), while melatonin was synthesized by MedChemExpress (Monmouth Junction, NJ, USA). .. Hydrochloric acid and sulfuric acid were sourced from Sinopharm Chemical Reagent Co., Ltd (Beijing, China).

Article Title: In vivo photopharmacology with light-activated opioid drugs
Article Snippet: Human embryonic kidney 293T (HEK293T) cells were grown in Complete DMEM (Dulbecco’s modified Eagle’s medium (Invitrogen, Carlsbad, CA) containing 5% fetal bovine serum (Corning), 50 U/mL Penicillin-Streptomycin (Invitrogen), and 1 mM sodium pyruvate (Corning)) and maintained at 37 °C in an atmosphere of 5% CO 2 in 10 cm TC dishes. .. Media in 10 cm TC dishes with HEK 293T cells (at around 70% confluence) were replaced with Opti-MEM (Invitrogen) followed by addition of a mixture of the GPCR plasmid, the GloSensor (Promega) 22F cAMP dependent reporter plasmid (Promega), and Lipofectamine 2000 (Invitrogen) in Opti-MEM. ..

Functional Assay:

Article Title: Prolonging parathyroid hormone analog action in vitro and in vivo through peptide lipidation
Article Snippet: .. Functional assays were performed in stably transfected cell lines derived from HEK-293 cells (ATCC Cat. # CRL-1573) and expressing the glosensor cAMP reporter (plasmid p22f, Promega) along with either the human PTH1R (GP-2.3 cells), pHL2-hPTH1R, in which pHluorin2, a pH-sensitive GFP variant, is inserted into the E2 region of the hPTH1R’s ECD (GPG-10 cells), PTH1R-delNt yfp , in which the receptor’s N-terminal extracellular domain (ECD) is replaced by yellow fluorescent protein (GD-5y cells), or β-Arrestin2 yfp (GBR-24 cells). .. Assays were also performed in SGS-72 cells, which are derived from the human osteoblastic cell line Saos-2 (ATCC Cat. # HTB-85) and stably express glosensor.

Stable Transfection:

Article Title: Prolonging parathyroid hormone analog action in vitro and in vivo through peptide lipidation
Article Snippet: .. Functional assays were performed in stably transfected cell lines derived from HEK-293 cells (ATCC Cat. # CRL-1573) and expressing the glosensor cAMP reporter (plasmid p22f, Promega) along with either the human PTH1R (GP-2.3 cells), pHL2-hPTH1R, in which pHluorin2, a pH-sensitive GFP variant, is inserted into the E2 region of the hPTH1R’s ECD (GPG-10 cells), PTH1R-delNt yfp , in which the receptor’s N-terminal extracellular domain (ECD) is replaced by yellow fluorescent protein (GD-5y cells), or β-Arrestin2 yfp (GBR-24 cells). .. Assays were also performed in SGS-72 cells, which are derived from the human osteoblastic cell line Saos-2 (ATCC Cat. # HTB-85) and stably express glosensor.

Transfection:

Article Title: Prolonging parathyroid hormone analog action in vitro and in vivo through peptide lipidation
Article Snippet: .. Functional assays were performed in stably transfected cell lines derived from HEK-293 cells (ATCC Cat. # CRL-1573) and expressing the glosensor cAMP reporter (plasmid p22f, Promega) along with either the human PTH1R (GP-2.3 cells), pHL2-hPTH1R, in which pHluorin2, a pH-sensitive GFP variant, is inserted into the E2 region of the hPTH1R’s ECD (GPG-10 cells), PTH1R-delNt yfp , in which the receptor’s N-terminal extracellular domain (ECD) is replaced by yellow fluorescent protein (GD-5y cells), or β-Arrestin2 yfp (GBR-24 cells). .. Assays were also performed in SGS-72 cells, which are derived from the human osteoblastic cell line Saos-2 (ATCC Cat. # HTB-85) and stably express glosensor.

Derivative Assay:

Article Title: Prolonging parathyroid hormone analog action in vitro and in vivo through peptide lipidation
Article Snippet: .. Functional assays were performed in stably transfected cell lines derived from HEK-293 cells (ATCC Cat. # CRL-1573) and expressing the glosensor cAMP reporter (plasmid p22f, Promega) along with either the human PTH1R (GP-2.3 cells), pHL2-hPTH1R, in which pHluorin2, a pH-sensitive GFP variant, is inserted into the E2 region of the hPTH1R’s ECD (GPG-10 cells), PTH1R-delNt yfp , in which the receptor’s N-terminal extracellular domain (ECD) is replaced by yellow fluorescent protein (GD-5y cells), or β-Arrestin2 yfp (GBR-24 cells). .. Assays were also performed in SGS-72 cells, which are derived from the human osteoblastic cell line Saos-2 (ATCC Cat. # HTB-85) and stably express glosensor.

Variant Assay:

Article Title: Prolonging parathyroid hormone analog action in vitro and in vivo through peptide lipidation
Article Snippet: .. Functional assays were performed in stably transfected cell lines derived from HEK-293 cells (ATCC Cat. # CRL-1573) and expressing the glosensor cAMP reporter (plasmid p22f, Promega) along with either the human PTH1R (GP-2.3 cells), pHL2-hPTH1R, in which pHluorin2, a pH-sensitive GFP variant, is inserted into the E2 region of the hPTH1R’s ECD (GPG-10 cells), PTH1R-delNt yfp , in which the receptor’s N-terminal extracellular domain (ECD) is replaced by yellow fluorescent protein (GD-5y cells), or β-Arrestin2 yfp (GBR-24 cells). .. Assays were also performed in SGS-72 cells, which are derived from the human osteoblastic cell line Saos-2 (ATCC Cat. # HTB-85) and stably express glosensor.

Article Title: Prolonging parathyroid hormone analog action in vitro and in vivo through peptide lipidation.
Article Snippet: .. # CRL-1573) and expressing the glosensor cAMP reporter (plasmid p22f, Promega) along with either the human PTH1R (GP-2.3 cells), pHL2-hPTH1R, in which pHluorin2, a pH-sensitive GFP variant, is inserted into the E2 region of the hPTH1R’s ECD (GPG-10 cells), PTH1R-delNtyfp, in which the receptor’s N-terminal extracellular domain (ECD) is replaced by yellow fluorescent protein (GD-5y cells), or β-Arrestin2yfp (GBR-24 cells). .. Assays were also performed in SGS-72 cells, which are derived from the humanosteoblastic cell line Saos-2 (ATCC Cat. # HTB-85) and stably express glosensor.

Synthesized:

Article Title: Pharmacological modulation of MRAP2 protein on murine melatonin receptor signaling
Article Snippet: .. The pGloSensor-22F cAMP plasmid and GloSensor cAMP reagent were procured from Promega (Madison, WI, USA), while melatonin was synthesized by MedChemExpress (Monmouth Junction, NJ, USA). .. Hydrochloric acid and sulfuric acid were sourced from Sinopharm Chemical Reagent Co., Ltd (Beijing, China).



Similar Products

90
Promega glosensor camp reporter plasmid p22f
Glosensor Camp Reporter Plasmid P22f, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glosensor+-22f+camp+plasmid/glosensor+camp+reporter+plasmid+p22f/pm40368898-308-5-10
Average 90 stars, based on 1 article reviews
glosensor camp reporter plasmid p22f - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
Yeasen Biotechnology camp biosensor plasmid glosensor
Camp Biosensor Plasmid Glosensor, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glosensor+-22f+camp+plasmid/camp+biosensor+plasmid+glosensor/pmc11437176-224-8-24
Average 90 stars, based on 1 article reviews
camp biosensor plasmid glosensor - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
Promega glosensor-camp plasmid
Glosensor Camp Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glosensor+-22f+camp+plasmid/pglosensor+22f+camp+plasmid/bio_rxiv__2024__05__01__591950-210-8-10
Average 90 stars, based on 1 article reviews
glosensor-camp plasmid - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
Promega glosensor -20f camp plasmid e1171
Glosensor 20f Camp Plasmid E1171, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glosensor+-22f+camp+plasmid/pglosensor+22f+camp+plasmid/pm38522511-257-13-18
Average 90 stars, based on 1 article reviews
glosensor -20f camp plasmid e1171 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
Promega glosensor camp plasmid
Glosensor Camp Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glosensor+-22f+camp+plasmid/pglosensor+22f+camp+plasmid/us11931374-530-19-26
Average 90 stars, based on 1 article reviews
glosensor camp plasmid - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
Promega camp-glosensor plasmid
Camp Glosensor Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glosensor+-22f+camp+plasmid/pglosensor+22f+camp+plasmid/us11931374-508-43-45
Average 90 stars, based on 1 article reviews
camp-glosensor plasmid - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
Promega glosensor camp reporter promega plasmid p22f
Glosensor Camp Reporter Promega Plasmid P22f, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glosensor+-22f+camp+plasmid/glosensor+camp+reporter+plasmid+p22f/pm38417010__ja3c09694_si_001-24-17-20
Average 90 stars, based on 1 article reviews
glosensor camp reporter promega plasmid p22f - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
Promega glosensor-22 f camp plasmid
Glosensor 22 F Camp Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glosensor+-22f+camp+plasmid/pglosensor+22f+camp+plasmid/pm37865997-54-10-12
Average 90 stars, based on 1 article reviews
glosensor-22 f camp plasmid - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results